Scientist Gene Therapy Vectorology

Company: Sanofi
Location: Framingham
Posted on: June 25, 2022

Job Description:

Position Overview:The Genomic Medicine Unit (GMU) CMC group at Sanofi is dedicated to the establishment of best in class manufacturing platforms to support development of life-changing advanced cell and gene therapy products, including recombinant AAV vectors, lentiviral vectors and cell therapy products.This organization encompasses vector and cell line engineering, upstream and downstream process development, analytics, and clinical manufacturing support.TheVectorology group is responsible forthe design, construction and QC of plasmids used for commercial production of recombinant AAV, as well as providing vectorology support to other efforts within the unit. We seek a highly motivated scientist with hands-on experience in molecular cloning, mammalian tissue culture and knowledge in AAV biology and molecular virology to join the team.Key Responsibilities:Independently design and construct viral vector and gene expression plasmids with minimal supervisionProduce and QC plasmids to meet sequence, purity and biosafety requirementsPerform mammalian tissue culture experiments (transfections, infections) to verify plasmid functionalityDevelop, maintain and demonstrate advanced knowledge of principles and techniques in molecular cloning, cell culture, AAV biology and viral vector productionCommunicate effectively with internal teams and external CROsDeliver plasmids on pipeline programs in compliance with aggressive timelinesKeep up to date on industry knowledge and advancements; provide input on optimization of process or capabilityManage plasmid inventory; provide input and appropriate reagents to assist different studiesWrite notebooks and protocols; author reports to support regulatory filings; present findings/results in multi-functional team meetingsMentor and support junior staff and build a culture of support and collaborationMaintain a functional and safe working environment while maximizing laboratory efficiencyBasic Qualifications:PhD in virology, molecular biology, microbiology or related biological sciences, or a Master's degree with a minimum of 4 years of relevant industry experience or a Bachelor's degree with a minimum of 6 years of relevant industry experienceStrong expertise in molecular cloning of vector plasmids (restriction digestion, ligation and screening), experience with AAV ITR plasmids a plus.Expertise in basic molecular biology techniques (DNA/RNA purification, qPCR, SDS-PAGE, westerns, ELISAs, etc)Skilled with primer design, Sanger sequencing and sequence analysis software (next generation sequencing software a plus)Good knowledge of principles and techniques in AAV biology and molecular virologyHands-on experience with mammalian tissue culture (adherent and suspension cells) and viral vector productionAbility to design complex cloning strategy independently with minimal guidance; ability to interpret results analytically and troubleshoot technical problemsPreferred Qualifications:Experience with next generation sequencing (Illumina or PacBio platform) and NGS data analysis preferredExcellent communication and presentation skills; excellent organization and attention to detail; critical and creative thinkerExperience with regulated mammalian gene expression systemsKnowledge of multiple viral vector expression platforms and industrial process developmentWorking knowledge of other molecular cloning methods, such as Gibson assembly, Golden Gate assembly or Ligation Independent Cloning methodSanofi Inc. and its U.S. affiliates are Equal Opportunity and Affirmative Action employers committed to a culturally diverse workforce. All qualified applicants will receive consideration for employment without regard to race; color; creed; religion; national origin; age; ancestry; nationality; marital, domestic partnership or civil union status; sex, gender, gender identity or expression; affectional or sexual orientation; disability; veteran or military status or liability for military status; domestic violence victim status; atypical cellular or blood trait; genetic information (including the refusal to submit to genetic testing) or any other characteristic protected by law.GD-SALI-SAPDNAt Sanofi R&D North America, we deliver meaningful solutions for patients. We transform science into breakthrough, best-in-class and first-in-class medicines and vaccines. We believe in creating a diverse and inclusive workforce - and workplace - which brings together the collective brainpower of over 2,000 colleagues and provides you with an exciting place to grow and develop. We set the bar high, and we deliver. Join us and together we will build on our trusted legacy of breakthroughs for society.As a healthcare company and a vaccine manufacturer, Sanofi has an important responsibility to protect individual and public health.This position will require individuals to be fully vaccinated against COVID-19 as part of your job responsibilities.At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.PDN-950f1d39-e763-4a21-8ad3-402838c01837

Keywords: Sanofi, Framingham , Scientist Gene Therapy Vectorology, Healthcare , Framingham, Massachusetts